Videos

Breast Milk and Powdered Infant Formula Storage Made Simple

What are the recommended storage, preparation, and handling practices for breast milk and powdered infant formula?

Developed by: ANHI Team

Publication Date: 16 September 2026

Summary:

This 5-minute educational resource provides a practical overview of safe breast milk storage, thawing, and powdered infant formula preparation and storage based on CDC-referenced guidance.  It is designed to help educate parents and caregivers on evidence-based practices that support infant safety and nutrition. 

The content begins with essential hygiene practices before expressing breast milk, including handwashing and ensuring breast pump components are clean.  The guide provides storage guidance for freshly expressed breast milk at room temperature, in the refrigerator, and in the freezer.  The resource also emphasizes proper storage containers, labeling practices, and strategies to minimize temperature fluctuations during storage. Additional content addresses safe thawing methods for frozen breast milk and recommendations for using thawed milk within specified timeframes. 

The resource also covers preparing and storing powdered infant formula.  Key topics include hand hygiene, cleaning and sanitizing feeding equipment, using safe water sources, following manufacturer mixing instructions precisely, and safe warming practices.  Guidance covers storage time limits for prepared formula, appropriate refrigeration, disposal of unfinished feedings, and storage of opened powdered formula containers. The guide also includes special considerations for infants at higher risk of infection. 

If you would like, you can download the Breast Milk and Powdered Infant Formula Storage Guide shown in this video.

Key Takeaways:
  • Freshly expressed or pumped breast milk should be stored for up to 4 hours at room temperature (77°F/25°C or lower), up to 4 days in a refrigerator (40°F/4°C), and is best used within 6 months when frozen, though acceptable for up to 12 months.
  • Use thawed breast milk within 24 hours if thawed in the refrigerator, and within 2 hours after warming or reaching room temperature.  Never refreeze thawed human milk.
  • Use prepared powdered infant formula within 2 hours of preparation, within 1 hour after feeding begins, or refrigerate immediately after preparation and use within 24 hours. Do not freeze prepared powdered infant formula.
Related Questions & Answers:
  • How long can freshly expressed breast milk be stored safely?
    You may store freshly expressed breast milk up to 4 hours at room temperature (77°F/25°C or lower), up to 4 days in a refrigerator maintained at 40°F/4°C, and in a freezer, where it is best used within 6 months but acceptable for up to 12 months.
  • What are the recommended methods for thawing frozen breast milk?
    You may thaw frozen breast milk in the refrigerator, in a container of lukewarm water, or under lukewarm running water.  Thawed milk should not be refrozen.
  • What is the safest way to prepare powdered infant formula?
    Caregivers should wash their hands, clean and sanitize preparation surfaces and feeding equipment, use water from a safe source, and carefully follow the manufacturer’s instructions for the water-to-powder ratio.  Using the correct mixing instructions helps ensure safety and appropriate nutrient delivery.
  • How long can prepared infant formula be kept before it should be discarded?
    Use prepared infant formula within 2 hours of preparation when stored at room temperature, and within 1 hour after feeding starts. You may use refrigerated formula within 24 hours. Discard any leftover formula from a feeding.
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References:
  • Centers for Disease Control and Prevention. Breast Milk Storage and Preparation. CDC. Updated August 14, 2026. Accessed May 1, 2026. https://www.cdc.gov/breastfeeding/breast-milk-preparation-and-storage/handling-breastmilk.html
  • Centers for Disease Control and Prevention. Infant Formula Preparation and Storage. CDC. Updated May 14, 2026. Accessed August 27, 2026. https://www.cdc.gov/infant-toddler-nutrition/formula-feeding/preparation-and-storage.html.
  • U.S. Food and Drug Administration. Handling Infant Formula Safely: What You Need to Know. FDA. Accessed May 1, 2026. https://www.fda.gov/food/buy-store-serve-safe-food/handling-infant-formula-safely-what-you-need-know.